Measurement

Part:BBa_J119400

Designed by: Avery Umphreys   Group: Eckdahl Lab   (2016-07-18)


tCloneTet+PDB riboswitch with P5 and BD10

The BD10 C Dog RBS has a lower binding efficiency than BD18. PCR Amplification was used to amplify the J100255 plasmid, excluding BD18, and BD10 was inserted via Decoupled Golden Gate Assembly.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found in sequence at 79
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 79
    Illegal NheI site found at 454
    Illegal NotI site found at 85
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 79
    Illegal BamHI site found at 600
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 79
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 79
    Illegal XbaI site found at 94
    Illegal NgoMIV site found at 626
  • 1000
    COMPATIBLE WITH RFC[1000]


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