Measurement
Part:BBa_J119400
Designed by: Avery Umphreys Group: Eckdahl Lab (2016-07-18)
tCloneTet+PDB riboswitch with P5 and BD10
The BD10 C Dog RBS has a lower binding efficiency than BD18. PCR Amplification was used to amplify the J100255 plasmid, excluding BD18, and BD10 was inserted via Decoupled Golden Gate Assembly.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal prefix found in sequence at 79
- 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 79
Illegal NheI site found at 454
Illegal NotI site found at 85 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 79
Illegal BamHI site found at 600 - 23INCOMPATIBLE WITH RFC[23]Illegal prefix found in sequence at 79
- 25INCOMPATIBLE WITH RFC[25]Illegal prefix found in sequence at 79
Illegal XbaI site found at 94
Illegal NgoMIV site found at 626 - 1000COMPATIBLE WITH RFC[1000]
[edit]
Categories
Parameters
None |